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Mfg Part Number

Ab00777-1.6-BS

Vector Labs Ab00777-1.6-BS Anti-alpha-2 integrin inserted-domain [2E10], Mouse Fab fragment, His-Tagged, kappa (1 mg)

VECTOR LABS

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Quick Overview

2E10 was generated by immunizing BALB/c mice with a DNA vaccine containing the human alpha2 I-domain cDNAsequence within pVAC2. The alpha2 I-domain is expressed as a fusion protein with fragment C of tetanus toxoid (FrC-TT). The scFv was then created using a V-gene phage display library derived from RNA from mice splenocytes and isolated following selection on GST-I after 3 rounds of panning.

$5,162.30

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Description

Product Class: Purified
Clone ID: 2E10
Heavy Chain Modification: His-Tagged
Synonyms: CD49b; alpha2-beta1 integrin; glycoprotein IaIIa; GP IaIIa; VLA2; VLA-2; collagen receptor; alpha-2 integrin I-domain; integrin alpha-2; integrin alpha 2
Amount: 1 mg
Application Codes Clone: ELISA; FC
Shipping Temperature: Wet Ice
Origin Pub PMID: 16411412
Buffer Composition: PBS only.
Original Format: IgG (scFv)
Storage Temperature: Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG (scFv) format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: scFv 2E10 can bind to the I (inserted) domain of recombinant alpha2 (GST- and His-), but cannot bind to the native epitope on platelets. This scFv also cross-reacts with and binds to the I-domain of alpha1 integrin. The alpha2 I-domain consists of ~200 amino acids and contains the ligand-binding site. The α2β1 integrin is expressed on platelets, fibroblasts, activated T lympho-cytes, endothelial cells and epithelial cells where its function is to act as a collagen and laminin receptor.
Application Notes: 2E10 scFv can be used to detect alpha2 and alpha1 intergin I-domain in ELISA and FC studies.

Product Class:	Purified
Clone ID:	2E10
Heavy Chain Modification:	His-Tagged
Synonyms:	CD49b; alpha2-beta1 integrin; glycoprotein IaIIa; GP IaIIa; VLA2; VLA-2; collagen receptor; alpha-2 integrin I-domain; integrin alpha-2; integrin alpha 2
Amount:	1 mg
Application Codes Clone:	ELISA; FC
Shipping Temperature:	Wet Ice
Origin Pub PMID:	16411412
Buffer Composition:	PBS only.
Original Format:	IgG (scFv)
Storage Temperature:	Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG (scFv) format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	scFv 2E10 can bind to the I (inserted) domain of recombinant alpha2 (GST- and His-), but cannot bind to the native epitope on platelets. This scFv also cross-reacts with and binds to the I-domain of alpha1 integrin. The alpha2 I-domain consists of ~200 amino acids and contains the ligand-binding site. The α2β1 integrin is expressed on platelets, fibroblasts, activated T lympho-cytes, endothelial cells and epithelial cells where its function is to act as a collagen and laminin receptor.
Application Notes:	2E10 scFv can be used to detect alpha2 and alpha1 intergin I-domain in ELISA and FC studies.

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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