Mfg Part Number
Ab00782-10.0
Vector Labs Ab00782-10.0 Anti-A33 [huA33], Human IgG1, kappa (200 μg)
Quick Overview
hA33 was generated by humanization of mouse A33 IgG2a-κ by CDR-grafting, and expressed in NSO cells. The mouse A33 antibody was prepared by immunizing BALB/c mice with AsPC-1 pancreatic adenocarcinoma cell line SP2/0 myeloma cells.
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| Product Class: | Purified |
|---|---|
| Clone ID: | huA33 |
| Synonyms: | hA33; A33; GPA33; glycoprotein A33; cell surface A33 antigen |
| Amount: | 200 μg |
| Application Codes Clone: | deplete; ELISA; IF; IHC |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 8519646 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | hA33 binds specifically to the ectodomain of A33 cell-surface differentation antigen. The antigen is a single-pass type-1 membrane glyoprotein of the immunoglobulin superfamily and, while not present in most normal and tumor cell type, it is expressed on the vast majority (95%) of both primary and metastatic colon cancer cells. The protein is involved in cell-cell recognition and signaling. |
| Application Notes: | The binding specificity and affinity of the humanized antibody has been shown to be equal to that of the orginal mouse antibody (1.3 nM), as is the uptake of 90Y-labelled antibody by human coloractal xenografts in nude mice. The circulating half-life of huA33 is also greater than that of the mouse antibody. Antigen-binding assays have been conducted by ELISA using Colo205 and SWI222 A33 antigen-expressing cells. Biodistribution assays have also been performed using hA33-12N4 conjugate in nude mice bearing subcutaneous SWI222 tumour xenografts. This antibody can be used as a biomarker for colorectal carcinoma.TFM (trivalent Fab fragment - constructed using trimaleimide cross-linkers) was shown to have a greater binding avidity for the target compared to huA33 with faster blood clearance shown in cynomolgus monkeys and guinea pigs. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |