Vector Labs Ab00782-10.6 Anti-A33 [huA33], Human Fab fragment, His-Tagged, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: huA33
Heavy Chain Modification: His-Tagged
Synonyms: hA33; A33; GPA33; glycoprotein A33; cell surface A33 antigen
Amount: 200 μg
Application Codes Clone: deplete; ELISA; IF; IHC
Shipping Temperature: Wet Ice
Origin Pub PMID: 8519646
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted human antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: hA33 binds specifically to the ectodomain of A33 cell-surface differentation antigen. The antigen is a single-pass type-1 membrane glyoprotein of the immunoglobulin superfamily and, while not present in most normal and tumor cell type, it is expressed on the vast majority (95%) of both primary and metastatic colon cancer cells. The protein is involved in cell-cell recognition and signaling.
Application Notes: The binding specificity and affinity of the humanized antibody has been shown to be equal to that of the orginal mouse antibody (1.3 nM), as is the uptake of 90Y-labelled antibody by human coloractal xenografts in nude mice. The circulating half-life of huA33 is also greater than that of the mouse antibody. Antigen-binding assays have been conducted by ELISA using Colo205 and SWI222 A33 antigen-expressing cells. Biodistribution assays have also been performed using hA33-12N4 conjugate in nude mice bearing subcutaneous SWI222 tumour xenografts. This antibody can be used as a biomarker for colorectal carcinoma.TFM (trivalent Fab fragment - constructed using trimaleimide cross-linkers) was shown to have a greater binding avidity for the target compared to huA33 with faster blood clearance shown in cynomolgus monkeys and guinea pigs.

Product Class:	Purified
Clone ID:	huA33
Heavy Chain Modification:	His-Tagged
Synonyms:	hA33; A33; GPA33; glycoprotein A33; cell surface A33 antigen
Amount:	200 μg
Application Codes Clone:	deplete; ELISA; IF; IHC
Shipping Temperature:	Wet Ice
Origin Pub PMID:	8519646
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted human antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	hA33 binds specifically to the ectodomain of A33 cell-surface differentation antigen. The antigen is a single-pass type-1 membrane glyoprotein of the immunoglobulin superfamily and, while not present in most normal and tumor cell type, it is expressed on the vast majority (95%) of both primary and metastatic colon cancer cells. The protein is involved in cell-cell recognition and signaling.
Application Notes:	The binding specificity and affinity of the humanized antibody has been shown to be equal to that of the orginal mouse antibody (1.3 nM), as is the uptake of 90Y-labelled antibody by human coloractal xenografts in nude mice. The circulating half-life of huA33 is also greater than that of the mouse antibody. Antigen-binding assays have been conducted by ELISA using Colo205 and SWI222 A33 antigen-expressing cells. Biodistribution assays have also been performed using hA33-12N4 conjugate in nude mice bearing subcutaneous SWI222 tumour xenografts. This antibody can be used as a biomarker for colorectal carcinoma.TFM (trivalent Fab fragment - constructed using trimaleimide cross-linkers) was shown to have a greater binding avidity for the target compared to huA33 with faster blood clearance shown in cynomolgus monkeys and guinea pigs.

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews