Mfg Part Number
Ab00788-23.0
Vector Labs Ab00788-23.0 Anti-Lymphotoxin alpha [3F12.2D3], Rabbit IgG, kappa (200 μg)
Quick Overview
3F12 was prepared by hyperimmunizing BALB/c mice with purified recombinant human LTα expressed in E. coli - the hybridoma clone was selected for its ability to bind LTα3 by ELISA. The CDRs of the antibody were grafted to the framework regions of the mice mAb 2G7 to create the chimeric version of the antibody.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 3F12.2D3 |
| Synonyms: | LTα3 chimeric 3F12;LT;; LTα; Tumor necrosis factor beta; Tumor necrosis factor β; Tumor necrosis factor-beta; tumor necrosis factor-β; TNF beta; TNF-beta; TNF β; TNF-β; Lymphotoxin-alpha; Lymphotoxin alpha |
| Amount: | 200 μg |
| Application Codes Clone: | neutralize; ELISA; FC; SPR |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 21268269 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | 3F12 binds to human LTα3 and LTαβ (with a KD of ~0.3 nM, determined by BIACORE; ~ 37 pM, determined by ELISA) in both its soluble homotrimeric and membrane heterotrimeric forms. LTα belongs to the tumor necrosis factor (TNF) superfamily and is secreted as a homo-trimer (LTα3), or is expressed on the cell surface in complex with LTβ. Lymphotoxin is produced by lymphocytes and in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. LTα3 signaling induces target cells to upregulate many chemokines and cytokines in an NFkB-dependent manner. |
| Application Notes: | The properties of the chimieric 3F12 is equivalent to that of the original 3F12 mAb. 3F12 is able to bind to Th2 cells and additionally is can functionally block human LTα3 versus an isotype control (trastuzumab) - LTα3-induced ICAM upregulation is blocked on HUVEC (Human Umbilical Endothelial Cells) (IC50 0.04 nM) and LTα3-induced IL-8 is blocked in A549 cells. The antibody has also been used in ELISA studies to detect LT-alpha and to measure binding. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |