Vector Labs Ab00792-23.0 Anti-IFNα [AIFN], Rabbit IgG, lambda (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: AIFN
Synonyms: interferon alpha; AIFNα1bScFv01; AIFNα1bIgG01; interferon α; interferon-α; IFNα1b; IFN-α1b; IFN-α; interferon α1b; interferon-α1b; IFN-alpha; INF alpha; interferon-alpha
Amount: 200 μg
Application Codes Clone: Neutralize
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: AIFNα1bScFv01 and the AIFNα1bIgG01 whole antibody bind to IFNα1b (KD ~0.75 nM determined by SPR); the epitope consists of the P26-N40 region in loop AB and E147-R150 region in helix E of IFNα1b - residues L30, D32, D35 and R150 are critical for binding. The antibody also binds to IFNR2, which shares partial epitopes with IFNα1b - binding affinity to IFNR2 is much weaker. The antibody does not bind to recombinant IFNα2b or IFNγ. IFNα is a cytokine and modulates the activtity of T cells, B cells and dendritic cells to regulate immunological functions, and are produced during the early stages of the immune respose. Overproduction of IFNα can result in the occurence of autoimmune diseases such as SLE (systemic lupus erythematosus), a multi-organ autoimmune disease that primarily affects the skin, joints, blood cells, heart, kidneys, and the nervous system.
Application Notes: AIFNα1bIgG01, the IgG1 version of the antibody, is able to cause downregulation of ISG15 and IFIT-1 expression ex vivo in PBMCs, induced by either recombinant IFNα1b or naiive IFNα from SLE patients’ sera. The antibody also reduces the total serum IgG and IgM antibody levels in a pristane-primed lupus-like mouse model. This neutralization occurs by out-competing IFNR2 for IFNα1b, which prevents the formation of the IFNAR2-IFNα interaction and the IFNα-mediated signaling pathway.

Product Class:	Purified
Clone ID:	AIFN
Synonyms:	interferon alpha; AIFNα1bScFv01; AIFNα1bIgG01; interferon α; interferon-α; IFNα1b; IFN-α1b; IFN-α; interferon α1b; interferon-α1b; IFN-alpha; INF alpha; interferon-alpha
Amount:	200 μg
Application Codes Clone:	Neutralize
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	AIFNα1bScFv01 and the AIFNα1bIgG01 whole antibody bind to IFNα1b (KD ~0.75 nM determined by SPR); the epitope consists of the P26-N40 region in loop AB and E147-R150 region in helix E of IFNα1b - residues L30, D32, D35 and R150 are critical for binding. The antibody also binds to IFNR2, which shares partial epitopes with IFNα1b - binding affinity to IFNR2 is much weaker. The antibody does not bind to recombinant IFNα2b or IFNγ. IFNα is a cytokine and modulates the activtity of T cells, B cells and dendritic cells to regulate immunological functions, and are produced during the early stages of the immune respose. Overproduction of IFNα can result in the occurence of autoimmune diseases such as SLE (systemic lupus erythematosus), a multi-organ autoimmune disease that primarily affects the skin, joints, blood cells, heart, kidneys, and the nervous system.
Application Notes:	AIFNα1bIgG01, the IgG1 version of the antibody, is able to cause downregulation of ISG15 and IFIT-1 expression ex vivo in PBMCs, induced by either recombinant IFNα1b or naiive IFNα from SLE patients’ sera. The antibody also reduces the total serum IgG and IgM antibody levels in a pristane-primed lupus-like mouse model. This neutralization occurs by out-competing IFNR2 for IFNα1b, which prevents the formation of the IFNAR2-IFNα interaction and the IFNα-mediated signaling pathway.

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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