Vector Labs Ab00795-23.0 Anti-Parathyroid Hormone-related Protein [23-57-137-1], Rabbit IgG, lambda (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 23-57-137-1
Synonyms: PTHrP; PTH-rP; Parathyroid hormone-like protein; PLP; PTHrP[1-34]
Amount: 200 μg
Application Codes Clone: neutralize; crystallize
Shipping Temperature: Wet Ice
Origin Pub PMID: 15517871
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: The anitbody binds to human PTHrP (The KD ~ 0.1 nM) at a site closely overlapping with the GPCR parathyroid hormone receptor 1 binding site - the epitope is between residues 20 and 30, with some contribution from residues 15-20 (aa 14-34 are important for receptor binding and aa1-34 are important for full signal tranduction). PTHrP is known to be a causative factor of humoural hypercalcemia of malignancy (HHM), which is frequently found at advanced stages of cancer. The protein binds to PTHR1, which is expressed on the surface of cells of the osteoblast lineage and of the kidney tubule; this interaction actively promotes bone resorption, in a manner identical to that of PTH.
Application Notes: The antibody blocks the binding of PTHrP to parathyroid hormone receptor 1 through binding to a site closely overlapping with the receptor-interacting site at the extracellular loop and juxtamembrane regions. The antibody does not block the action of parathyroid hormone (PTH) through the same receptor. The antibody inhibits the action of PTHrP-(1–34) in promoting cAMP production by osteosarcoma cells by 90%,. The antibody also functions in vivo, and can treat nude mice rendered hypercalcemic by PAN-7-JCK cells, and prevents cachexia in mice bearing the LC6-JCK human lung cancer xenograft, independent of the calcium-lowering effect of the antibody. The fab fragment of this antibody, generated by papain digestion, has been used for cystallization of PTHrP at atomic level resolution.

Product Class:	Purified
Clone ID:	23-57-137-1
Synonyms:	PTHrP; PTH-rP; Parathyroid hormone-like protein; PLP; PTHrP[1-34]
Amount:	200 μg
Application Codes Clone:	neutralize; crystallize
Shipping Temperature:	Wet Ice
Origin Pub PMID:	15517871
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	The anitbody binds to human PTHrP (The KD ~ 0.1 nM) at a site closely overlapping with the GPCR parathyroid hormone receptor 1 binding site - the epitope is between residues 20 and 30, with some contribution from residues 15-20 (aa 14-34 are important for receptor binding and aa1-34 are important for full signal tranduction). PTHrP is known to be a causative factor of humoural hypercalcemia of malignancy (HHM), which is frequently found at advanced stages of cancer. The protein binds to PTHR1, which is expressed on the surface of cells of the osteoblast lineage and of the kidney tubule; this interaction actively promotes bone resorption, in a manner identical to that of PTH.
Application Notes:	The antibody blocks the binding of PTHrP to parathyroid hormone receptor 1 through binding to a site closely overlapping with the receptor-interacting site at the extracellular loop and juxtamembrane regions. The antibody does not block the action of parathyroid hormone (PTH) through the same receptor. The antibody inhibits the action of PTHrP-(1–34) in promoting cAMP production by osteosarcoma cells by 90%,. The antibody also functions in vivo, and can treat nude mice rendered hypercalcemic by PAN-7-JCK cells, and prevents cachexia in mice bearing the LC6-JCK human lung cancer xenograft, independent of the calcium-lowering effect of the antibody. The fab fragment of this antibody, generated by papain digestion, has been used for cystallization of PTHrP at atomic level resolution.

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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