Mfg Part Number
Ab00798-23.0
Vector Labs Ab00798-23.0 Anti-Hemagglutinin 5 [H5.3], Rabbit IgG, lambda (200 μg)
Quick Overview
H5.3 was generated from hybridomas created by fusing PBMCs (peripheral blood mononuclear cells) from human donors who had been previously received an A/Vietnam/1203/2004 H5N1 subunit vaccine with myeloma cells. The hybridoma cells were screened for those showing binding affinity for H5 HA.
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| Product Class: | Purified |
|---|---|
| Clone ID: | H5.3 |
| Synonyms: | H5 HA |
| Amount: | 200 μg |
| Application Codes Clone: | Neutralize |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | H5.3 binds to H5 at the head domain at an epitope which involves the 190 loop. The epitope was determined to be LGVSSA (aa 134-139) and YQGKSS (aa 142-147) of the head domain by DXMS (deuterium exchange mass spectrometry). This antibody shows reactivity with the wild-type H5N1 field strains VN/1203 (EC50 ~ 17 ng/ml), HK/156 (EC50 ~ 18 ng/ml), Anhui (EC50 ~ 2.8 ug/ml) and Indo (EC50 ~ 10 ug/ml). HA is a viral glycoprotein initially assembled as a trimer on the viral envelope as the preprotein HA0. It is subsequently cleaved by host proteases. |
| Application Notes: | H5.3 binds to H5 where it is able to block the receptor binding site on HA by inserting its CDR3 into the site (IC50 ~ 20 ng/ml). H5.3 can neutralize wild-type VN/1203, rdt VN/1203 K and VN/1203 F pseudovirions, all with IC50s of 5 ng/ml. This antibody also has potent HI (hemagglutination inhibition) activity (80 ng/ml). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |