Mfg Part Number
Ab00800-8.1
Vector Labs Ab00800-8.1 Anti-single-stranded poly(rI) RNA [Jel 103], Rat IgG2b, kappa (200 μg)
Quick Overview
Jel 103 was selected from a hybridoma cell line generated by the fusion of the MOPC 315-43 myeloma cell line with mouse spleen cells derived from C57/B1 mice which had been immunized with poly(rI)poly(dC) duplex complexed to methylated bovine serum albumin.
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| Product Class: | Purified |
|---|---|
| Clone ID: | Jel 103 |
| Synonyms: | polyriboinosinic acid; single stranded RNA; single-stranded RNA; inosine |
| Amount: | 200 μg |
| Application Codes Clone: | SPRIA; IF |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 7523684 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | Jel 103 is highly specific for single-stranded RNA and shows sequence specificity as it binds to poly(rI) but not to poly(dI), poly(rG) or to any other homopolymer - it does not bind randomly to calf thymus DNA. Jel 103 Fab is shown to form base stacking interactions with a Tyr residue and two salt-bridge interactions to the phosphate backbone with Lys and Arg residues. |
| Application Notes: | Jel 301 can be used for the detection of single-stranded poly(rI) and for binding studies where the antibody has been used previosly in solid-phase radioimmunoassays. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |