Mfg Part Number
Ab00805-1.1
Vector Labs Ab00805-1.1 Anti-HIV-1 protease [F11.2.32], Mouse IgG1, kappa (200 μg)
Quick Overview
F11.2.32 was prepared by generating a hybridoma cell line in which myeloma cells were fused with splenocytes derived from BALB/c mice immunized with HIV-1 PR in the presence of Freund's adjuvant. F11.2.32 was selected for its ability to bind to HIV-1 PR by ELISA.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | F11.2.32 |
| Synonyms: | HIV-1 PR; HIV1 PR; HIV1 protease |
| Amount: | 200 μg |
| Application Codes Clone: | neutralize; ELISA |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 9150407 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | F11.2.32 Fab binds to HIV-1 PR with a dissociation constant of 5 nM (determined by SPR at pH5.2) at an epitope consisting of MSLPGRWKPKM (aa 36-46). HIV-1 PR is a homodimeric apartate protease that specifically cleaves the viral Gag and Gag/Pol polyprotein precursor. A correlation has been observed between the activity of the protease and the degree of infectivity, and so this protein is a major antiviral target. |
| Application Notes: | The IgG and Fab variants of F11.2.32 are both able to strongly inhibit the catalytic activity of HIV-1 PR (IC50 ~35 nM). Inhibition occurs by distorting the native structure of the HIV-1 PR at the base of the flap region, which plays an important role in regulating access to the active site. This antibody is also able to detect HIV-1 PR by ELISA. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |