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Mfg Part Number

Ab00805-1.6-BS

Vector Labs Ab00805-1.6-BS Anti-HIV-1 protease [F11.2.32], Mouse Fab fragment, His-Tagged, kappa (1 mg)

VECTOR LABS

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Quick Overview

F11.2.32 was prepared by generating a hybridoma cell line in which myeloma cells were fused with splenocytes derived from BALB/c mice immunized with HIV-1 PR in the presence of Freund's adjuvant. F11.2.32 was selected for its ability to bind to HIV-1 PR by ELISA.

$5,162.30

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Description

Product Class: Purified
Clone ID: F11.2.32
Heavy Chain Modification: His-Tagged
Synonyms: HIV-1 PR; HIV1 PR; HIV1 protease
Amount: 1 mg
Application Codes Clone: neutralize; ELISA
Shipping Temperature: Wet Ice
Origin Pub PMID: 9150407
Buffer Composition: PBS only.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: F11.2.32 Fab binds to HIV-1 PR with a dissociation constant of 5 nM (determined by SPR at pH5.2) at an epitope consisting of MSLPGRWKPKM (aa 36-46). HIV-1 PR is a homodimeric apartate protease that specifically cleaves the viral Gag and Gag/Pol polyprotein precursor. A correlation has been observed between the activity of the protease and the degree of infectivity, and so this protein is a major antiviral target.
Application Notes: The IgG and Fab variants of F11.2.32 are both able to strongly inhibit the catalytic activity of HIV-1 PR (IC50 ~35 nM). Inhibition occurs by distorting the native structure of the HIV-1 PR at the base of the flap region, which plays an important role in regulating access to the active site. This antibody is also able to detect HIV-1 PR by ELISA.

Product Class:	Purified
Clone ID:	F11.2.32
Heavy Chain Modification:	His-Tagged
Synonyms:	HIV-1 PR; HIV1 PR; HIV1 protease
Amount:	1 mg
Application Codes Clone:	neutralize; ELISA
Shipping Temperature:	Wet Ice
Origin Pub PMID:	9150407
Buffer Composition:	PBS only.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	F11.2.32 Fab binds to HIV-1 PR with a dissociation constant of 5 nM (determined by SPR at pH5.2) at an epitope consisting of MSLPGRWKPKM (aa 36-46). HIV-1 PR is a homodimeric apartate protease that specifically cleaves the viral Gag and Gag/Pol polyprotein precursor. A correlation has been observed between the activity of the protease and the degree of infectivity, and so this protein is a major antiviral target.
Application Notes:	The IgG and Fab variants of F11.2.32 are both able to strongly inhibit the catalytic activity of HIV-1 PR (IC50 ~35 nM). Inhibition occurs by distorting the native structure of the HIV-1 PR at the base of the flap region, which plays an important role in regulating access to the active site. This antibody is also able to detect HIV-1 PR by ELISA.

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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