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Mfg Part Number

Ab00815-10.3

Vector Labs Ab00815-10.3 Anti-S100A8 & S100A9 [5.5], Human IgG1, Fc Silent™, kappa (200 μg)

VECTOR LABS

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Quick Overview

BALB/c mice were immunized using cells from acute monocytic leukemia patients. The BALB/c spleen cell suspension was fused with Sp2/0-Ag14 cells, and mAb 5.5 was isolated from the hybridoma cells either by radioimmunoassay with 125-I-labelled F(ab')2 fragments of sheep anti-mouse Ig as second layer, or by indirect immunofluorescence using rhodamine-conjugated goat anti-mouse Ig as the second layer.

$517.40

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Description

Product Class: Purified
Clone ID: 5.5
Heavy Chain Modification: Fc Silent™
Synonyms: MRP-8; MRP8; MRP-14; MRP14; CF antigen; CFAG; L1 molecule; p8,14; Calprotectin
Amount: 200 μg
Application Codes Clone: ELISA; FC; IHC; IP; WB
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: mAb 5.5 specifically recognises complexed MRP-8/MRP-14 via the hydrophobic residues in helix IV of MRP-8 (S100A8) exposed during heterodimer formation. mAb recognition of MRP-8 & MRP-14 is calcium dependent. 5.5 epitope is most likely to be formed by linear sequence Phe26-His27-Ala28 in calcium-binding loop I of MRP-8, and by Phe68, Leu72, Ile76 and Lys77 that lie along one face of helix IV.
Application Notes: mAb 5.5 has been used in immunoprecipitation and western blot experiments for the characterisation of p8,14 identified in extracts of monocytes and neutrophils. 5.5 was also used in flow cytometric analysis to show that p8,14 is not a cell surface protein, and in immunohistochemical staining to show that p8,14 is present extracellularly (Edgeworth 1991).

Product Class:	Purified
Clone ID:	5.5
Heavy Chain Modification:	Fc Silent™
Synonyms:	MRP-8; MRP8; MRP-14; MRP14; CF antigen; CFAG; L1 molecule; p8,14; Calprotectin
Amount:	200 μg
Application Codes Clone:	ELISA; FC; IHC; IP; WB
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	mAb 5.5 specifically recognises complexed MRP-8/MRP-14 via the hydrophobic residues in helix IV of MRP-8 (S100A8) exposed during heterodimer formation. mAb recognition of MRP-8 & MRP-14 is calcium dependent. 5.5 epitope is most likely to be formed by linear sequence Phe26-His27-Ala28 in calcium-binding loop I of MRP-8, and by Phe68, Leu72, Ile76 and Lys77 that lie along one face of helix IV.
Application Notes:	mAb 5.5 has been used in immunoprecipitation and western blot experiments for the characterisation of p8,14 identified in extracts of monocytes and neutrophils. 5.5 was also used in flow cytometric analysis to show that p8,14 is not a cell surface protein, and in immunohistochemical staining to show that p8,14 is present extracellularly (Edgeworth 1991).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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