Mfg Part Number
Ab00821-2.3
Vector Labs Ab00821-2.3 Anti-CD14 [UCH-M1], Mouse IgG2a, Fc Silent™, kappa (200 μg)
Quick Overview
Human thymocytes followed by peripheral blood mononuclear cells.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | UCH-M1 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | UCHM1; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein |
| Amount: | 200 μg |
| Application Codes Clone: | IHC; IF; WB |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for human CD14, which is expressed strongly by macrophages and most tissue monocytes. It cross-reacts with non-human primate and pig CD14. |
| Application Notes: | This antibody has been used to specifically stain monocytes and macrophages in cryostat sections of large cell lymphoma using an indirect immunoperoxidase technique (Addis et al, 1986) and cryostat lymph node sections (Salisbury et al, 1985). It has also been used to stain acetone-fixed cytocentrifuge preparations of a cell line derived from a patient with Hodgkin's disease (Jones et al, 1985). This antibody has been employed in an immunofluorescence analysis to assess CD14 expression in porcine epithelial cells (Liu et al, 2001), and in indirect immunofluorescence staining of cryostat thymus sections (Marinova et al, 2008). This antibody has also been used in Western blotting, and to deplete soluble CD14 from human sera through an immunoadsorption method (Shimizu et al, 2004). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |