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Mfg Part Number

Ab00849-13.12

Vector Labs Ab00849-13.12 Anti-CD29 [12G10], Human IgG4-S228P, kappa (200 μg)

VECTOR LABS

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Quick Overview

This antibody was raised by immunising BALB/C mice with purified β1 integrins from HT-1080 fibrosarcoma cells

$517.40

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Description

Product Class: Purified
Clone ID: 12G10
Synonyms: Integrin beta-1; Integrin β1 chain; Integrin β1; VLA-β chain; Glycoprotein Iia; GPIIA; Fibronectin receptor subunit beta; ITGB1; VLA-4 subunit beta; FNRB; MDF2; MSK12
Amount: 200 μg
Application Codes Clone: ELISA; FC; WB; IHC; therapeutic; enhance
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody recognises a ligand-induced binding site on the βA domain of β1 integrin, and thus reacts preferentially with α5β1 integrins in the ligand-activated state. Binding of this antibody alters the position of the α1 helix of β1 integrin, and results in further integrin activation.
Application Notes: This antibody reacts with β1 integrins in Western blotting (Mould et al, 1995). ELISA analysis has revealed the ability of this antibody to enhance the interaction between β1 integrin and its ligand, fibronectin (Mould et al, 1995). Indeed, activation of integrins by this antibody reduced the induction of osteosarcoma growth and inhibition of NF-κB caused by hERG1 knockdown in osteosarcoma cells (Zeng et al, 2016). This antibody has been used to specifically label ligand-bound cell surface β1 integrins both in immunohistochemical analysis (Izumi et al, 2015; Chao & Kunz, 2009), including in situ proximity ligation (PLA) analysis (Nevo et al, 2010), and in FACS analysis (Brandt et al, 2009).

Product Class:	Purified
Clone ID:	12G10
Synonyms:	Integrin beta-1; Integrin β1 chain; Integrin β1; VLA-β chain; Glycoprotein Iia; GPIIA; Fibronectin receptor subunit beta; ITGB1; VLA-4 subunit beta; FNRB; MDF2; MSK12
Amount:	200 μg
Application Codes Clone:	ELISA; FC; WB; IHC; therapeutic; enhance
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody recognises a ligand-induced binding site on the βA domain of β1 integrin, and thus reacts preferentially with α5β1 integrins in the ligand-activated state. Binding of this antibody alters the position of the α1 helix of β1 integrin, and results in further integrin activation.
Application Notes:	This antibody reacts with β1 integrins in Western blotting (Mould et al, 1995). ELISA analysis has revealed the ability of this antibody to enhance the interaction between β1 integrin and its ligand, fibronectin (Mould et al, 1995). Indeed, activation of integrins by this antibody reduced the induction of osteosarcoma growth and inhibition of NF-κB caused by hERG1 knockdown in osteosarcoma cells (Zeng et al, 2016). This antibody has been used to specifically label ligand-bound cell surface β1 integrins both in immunohistochemical analysis (Izumi et al, 2015; Chao & Kunz, 2009), including in situ proximity ligation (PLA) analysis (Nevo et al, 2010), and in FACS analysis (Brandt et al, 2009).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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