Mfg Part Number
Ab00854-1.1
Vector Labs Ab00854-1.1 Anti-IgG [NH3/130.5.2], Mouse IgG1, kappa (200 μg)
Quick Overview
This antibody was raised by immunising Balb/c mice with whole IgG fraction and subsequent hybridoma cells formation.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | NH3/130.5.2 |
| Synonyms: | immunoglobulin G; human immunoglobulin; NH3/130 |
| Amount: | 200 μg |
| Application Codes Clone: | WB; IP |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody recognises human IgG. The antibody cross-reacts with rhesus macaque IgG1, cynomolgus monkey IgG1 and cynomolgus monkey IgG4. |
| Application Notes: | This antibody was shown to have a high avidity to human immunoglobulin G having a high titre, equal to a corresponding rabbit polyclonal antibody, when tested against anti-D sensitised cells (Downie et al., 1983). NH3/130.5.2 detects human IgG samples transferred from the SDS polyacrylamide gel via Western blot under non-reducing conditions (Thorpe et al., 1984). Thorne et al. (1984) mentions that this antibody was also successful in immunoprecipitation in agar or solution (M. Spitz, unpublished results). Further, the antibody was capable of detecting IgGs via Western blot from native gels run under acidic conditions (Thorne et al., 1984). Finally, the antibody cross-reacted with rhesus macaque IgG1, cynomolgus monkey IgG1 and cynomolgus monkey IgG4 as shown by ELISA analysis. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |