Mfg Part Number
Ab00855-1.1

Vector Labs Ab00855-1.1 Anti-kappa light chain [NH3/41.34], Mouse IgG1, kappa (200 μg)

Quick Overview
This antibody was raised by immunising Balb/c mice with whole IgG fraction and subsequent hybridoma cells formation.
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Product Class:Purified
Clone ID:NH3/41.34
Synonyms:kappa Lc; human immunoglobulin kappa light chain; kappa chain; immunoglobulin kappa light chain; NH3/41
Amount:200 μg
Application Codes Clone:WB
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG1
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:This antibody recognises human immunoglobulin kappa light chain. The antibody does not cross react with rhesus macaque IgG1, cynomolgus monkey IgG1 and cynomolgus monkey IgG4.
Application Notes:NH3/41.34 antibody detects kappa light chains from a human IgGs and Bence-Jones protein samples via Western blot under reducing conditions (Thorpe et al., 1984). This antibody is equally able to detect full IgGs via Western blot when the samples are transferred from the non-reducing SDS polyacrylamide gels (Thorpe et al., 1984). Further, this antibody was capable of detecting IgGs via Western blot from native gels run under acidic conditions (Thorne et al., 1984). Finally, the specificity of the antibody for human IgG1 was confirmed by ELISA analysis. No cross reactivity with rhesus macaque IgG1, cynomolgus monkey IgG1 and cynomolgus monkey IgG4 was detected.
More Information
Brand Vector Labs
UNSPSC 12161500