Mfg Part Number
Ab00862-23.0
Vector Labs Ab00862-23.0 Anti-5-Hydroxymethylcytosine [AB3/63.3], Rabbit IgG, kappa (200 μg)
Quick Overview
5-Hydroxymethylcytosine conjugated to BSA.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | AB3/63.3 |
| Synonyms: | 5hmc; MABE176; 5hmeC; 5' hydroxymethyl; cytosine; 5'OH methyl cytosine; 5'OHmeC; 5hmc; 5-hmC |
| Amount: | 200 μg |
| Application Codes Clone: | IF; Dot; IP |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine. |
| Application Notes: | This antibody can be used to detect 5hmC by immunofluorescence and dot blot. Zhang et al. (2014, PMID: 24427299) used the original rat IgG2a format (under the name MABE176) in an immunoprecipitation experiment. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |