Mfg Part Number
Ab00864-23.0
Vector Labs Ab00864-23.0 Anti-TK15 epitope tag [TK 15], Rabbit IgG, kappa (200 μg)
Quick Overview
Mice were injected with bacterially expressed full-length Xenopus laevis Orc1p, and spleen cells from three best-responding mice were used to generate antibody-producing hybridomas. Antibodies were screened by enzyme-linked immunosorbent assay and immunoprecipitation.
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| Product Class: | Purified |
|---|---|
| Clone ID: | TK 15 |
| Synonyms: | TK-15 tag |
| Amount: | 200 μg |
| Application Codes Clone: | IF; IP; WB |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | Recognises recombinant proteins TK15 tag at the carboxy terminus. Amino terminal and internal TK15 tags have not been tested. |
| Application Notes: | This antibody was first used to isolate Xenopus origin recognition complex peptides (Tugal 1998) but can now be used for the purification and detection of recombinant fusion proteins incorporating a single eight amino acid TK15 tag. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |