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Mfg Part Number

Ab00870-23.0

Vector Labs Ab00870-23.0 Anti-Factor VIII [RFF-VIII:c/8], Rabbit IgG, kappa (200 μg)

VECTOR LABS

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Quick Overview

This antibody was raised by immunising BALB/C mice with highly purified procoagulant factor VIII (VIII: C). Spleen cells were then isolated from immunised mice, and fused with P3 NSI mouse myeloma cells to generate hybridomas.

$517.40

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Description

Product Class: Purified
Clone ID: RFF-VIII:c/8
Synonyms: AHF; Antihemophilic factor; coagulation factor VIII; procoagulant component; coagulation factor VIIIc; DXS1253E; F8C; factor VIII F8B; FVIII; HEMAF8B; Procoagulant component
Amount: 200 μg
Application Codes Clone: block; depletion; WB; column chromatography
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for human factor VIII, a glycoprotein involved in blood coagulation. Factor VIII acts as a cofactor for factor IXa which converts factor X to the activated form Xa in the presence of calcium and phospholipids. Defects in the F8 gene, which encodes Factor VIII, result in the coagulation disorder haemophilia A.
Application Notes: This antibody is a potent coagulation inhibitor (Rotblat, 1983).This antibody has been employed to produce factor VIII-deficient plasma by immunodepletion (Takase et al, 1987); purified monoclonal antibodies were bound to Sepharose columns, which were used to reduce the factor VII content of plasma through column chromatography. This antibody does not bind antigens if CRM negative haemophilic plasma, which lacks factor VIII, is used in column chromatography (Rotblat et al, 1983). This antibody has also been used in Western blotting to identify factor VIII (Rotblat et al, 1985)

Product Class:	Purified
Clone ID:	RFF-VIII:c/8
Synonyms:	AHF; Antihemophilic factor; coagulation factor VIII; procoagulant component; coagulation factor VIIIc; DXS1253E; F8C; factor VIII F8B; FVIII; HEMAF8B; Procoagulant component
Amount:	200 μg
Application Codes Clone:	block; depletion; WB; column chromatography
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for human factor VIII, a glycoprotein involved in blood coagulation. Factor VIII acts as a cofactor for factor IXa which converts factor X to the activated form Xa in the presence of calcium and phospholipids. Defects in the F8 gene, which encodes Factor VIII, result in the coagulation disorder haemophilia A.
Application Notes:	This antibody is a potent coagulation inhibitor (Rotblat, 1983).This antibody has been employed to produce factor VIII-deficient plasma by immunodepletion (Takase et al, 1987); purified monoclonal antibodies were bound to Sepharose columns, which were used to reduce the factor VII content of plasma through column chromatography. This antibody does not bind antigens if CRM negative haemophilic plasma, which lacks factor VIII, is used in column chromatography (Rotblat et al, 1983). This antibody has also been used in Western blotting to identify factor VIII (Rotblat et al, 1985)

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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