Vector Labs Ab00877-8.4 Anti-MHC II [P7/7], Rat IgG2b, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: P7/7
Heavy Chain Modification: Fc Silent™
Synonyms: P7.7; I-A; Ia; I-E; MHC-II; MHC Class II
Amount: 200 μg
Application Codes Clone: IHC; IP; FC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody recognises the rat RT1Bu/Du MHC class II antigen, and cross-reacts with haplotypes c, d, l and n. This antibody stains MHC class II molecules in a number of rat strains, including PVG, F334, LEW, and BN. However, this antibody is not reactive with RT1a haplotype rats, including Wistar rats. This antibody also cross-reacts with murine I-A and I-E molecules of all haplotypes tested, including H-2b, d, f and k, so can be used in all mouse strains.
Application Notes: This antibody reacts with murine MHC class II I-A and I-E molecules, as confirmed by two-dimensional gel electrophoresis of Ia immunoprecipitates from metabolically labelled murine lymphoma cells (Momburg et al, 1986). Cross-blocking studies with anti-Ia β-chain MAbs indicate that P7/7 binds to the β-chain of murine Ia (Momburg et al, 1986). This antibody has been used to stain acetone-fixed mouse pancreas sections to study lymphocyte infiltration (Signore et al, 1987; Signore et al, 1989) and ethanol-fixed mouse brain sections (McMenamin et al, 1998). In flow cytometric analysis, this antibody has been employed to identify MHC class II-expressing murine cells (Hayball & Lake, 2004; Heath et al, 1991), and to analyse the surface phenotype of murine N9 microglial cells (Dimayuga et al, 2005). This antibody has been used in immunofluorescence analysis of acetone-fixed thymus sections to identify MHC-II positive and negative medullae (Aschenbrenner et al, 2007).

Product Class:	Purified
Clone ID:	P7/7
Heavy Chain Modification:	Fc Silent™
Synonyms:	P7.7; I-A; Ia; I-E; MHC-II; MHC Class II
Amount:	200 μg
Application Codes Clone:	IHC; IP; FC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody recognises the rat RT1Bu/Du MHC class II antigen, and cross-reacts with haplotypes c, d, l and n. This antibody stains MHC class II molecules in a number of rat strains, including PVG, F334, LEW, and BN. However, this antibody is not reactive with RT1a haplotype rats, including Wistar rats. This antibody also cross-reacts with murine I-A and I-E molecules of all haplotypes tested, including H-2b, d, f and k, so can be used in all mouse strains.
Application Notes:	This antibody reacts with murine MHC class II I-A and I-E molecules, as confirmed by two-dimensional gel electrophoresis of Ia immunoprecipitates from metabolically labelled murine lymphoma cells (Momburg et al, 1986). Cross-blocking studies with anti-Ia β-chain MAbs indicate that P7/7 binds to the β-chain of murine Ia (Momburg et al, 1986). This antibody has been used to stain acetone-fixed mouse pancreas sections to study lymphocyte infiltration (Signore et al, 1987; Signore et al, 1989) and ethanol-fixed mouse brain sections (McMenamin et al, 1998). In flow cytometric analysis, this antibody has been employed to identify MHC class II-expressing murine cells (Hayball & Lake, 2004; Heath et al, 1991), and to analyse the surface phenotype of murine N9 microglial cells (Dimayuga et al, 2005). This antibody has been used in immunofluorescence analysis of acetone-fixed thymus sections to identify MHC-II positive and negative medullae (Aschenbrenner et al, 2007).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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