Mfg Part Number
Ab00879-2.0
Vector Labs Ab00879-2.0 Anti-MHC I [R1-9.6], Mouse IgG2a, kappa (200 μg)
Quick Overview
This antibody was raised by immunising Lewis 1F rats with mouse CH-1 lymphoma cells. Spleen cells from immunised rats were fused with X-63Ag8-653 myeloma cells to generate hybridomas.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | R1-9.6 |
| Synonyms: | R1.9.6; H-2; MHC-I; MHC Class I |
| Amount: | 200 μg |
| Application Codes Clone: | IP; IF; radioimmunoassay |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for murine MHC class I, and binds to polymorphic H-2 determinants expressed on most haplotypes. This antibody is, therefore, able to bind all H-2 class I molecules, independent of the allele. This antibody recognises a determinant distinct from those detected by most common mouse monoclonal anti-H-2 antibodies. |
| Application Notes: | This antibody binds murine H-2 MHC class I molecules and has been used in both structural and biochemical studies. This antibody has been used in immunoprecipitation analysis (Robinson, 1987) and in immunofluorescence assays for MHC-I surface expression (Karacsonyi et al., 2004) and MHC-I endocytosis (Knorr et al., 2009). This antibody has also been employed to induce clustering of cell surface MHC-I in co-patching assays (Knorr et al., 2009). Cellular radioimmune assays have been used to confirm the binding of this antibody to spleen cells and assess its strain specificity (Koch et al., 1983; PMID: 6192569). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |