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Mfg Part Number

Ab00880-24.2

Vector Labs Ab00880-24.2 Anti-MHC I [R1-21.2], Goat IgG, kappa (100 μg)

VECTOR LABS

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Quick Overview

This antibody was raised by immunising Lewis 1F rats with mouse CH-1 lymphoma cells. Spleen cells from immunised rats were fused with X-63Ag8-653 myeloma cells to generate hybridomas.

$570.70

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Description

Product Class: Purified
Clone ID: R1-21.2
Synonyms: R1.21.2; H-2; MHC-I; MHC Class I
Amount: 100 μg
Application Codes Clone: IHC; IP; radioimmunoassay; WB
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric goat antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for murine MHC class I, and binds to polymorphic H-2 determinants expressed on most haplotypes. This antibody is, therefore, able to bind all H-2 class I molecules, independent of the allele. This antibody inhibits the binding of most mouse anti-H-2 antibodies to H-2k cells.
Application Notes: This antibody binds murine H-2 MHC class I molecules, and has been used in both structural and biochemical studies. It has been employed to assess MHC class I expression in frozen, acetone-fixed mouse brain sections by immunohistochemistry (Miralvès et al, 2007), and to stain paraffin-embedded sections of mouse liver for H-2Kb expression (Limmer et al, 2005). It has also been used to precipitate mouse H-2Kk and H-2Dk class I molecules from detergent lysates of Ltk3.3/1 cells (Rada et al, 1990), and to stain H-2Kb and H-2Kk-expressing cells in FACS analysis (Bendelac, 1995). Cellular radioimmune assays have been used to confirm the binding of this antibody to spleen cells, and assess its strain specificity (Koch et al, 1983).

Product Class:	Purified
Clone ID:	R1-21.2
Synonyms:	R1.21.2; H-2; MHC-I; MHC Class I
Amount:	100 μg
Application Codes Clone:	IHC; IP; radioimmunoassay; WB
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric goat antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for murine MHC class I, and binds to polymorphic H-2 determinants expressed on most haplotypes. This antibody is, therefore, able to bind all H-2 class I molecules, independent of the allele. This antibody inhibits the binding of most mouse anti-H-2 antibodies to H-2k cells.
Application Notes:	This antibody binds murine H-2 MHC class I molecules, and has been used in both structural and biochemical studies. It has been employed to assess MHC class I expression in frozen, acetone-fixed mouse brain sections by immunohistochemistry (Miralvès et al, 2007), and to stain paraffin-embedded sections of mouse liver for H-2Kb expression (Limmer et al, 2005). It has also been used to precipitate mouse H-2Kk and H-2Dk class I molecules from detergent lysates of Ltk3.3/1 cells (Rada et al, 1990), and to stain H-2Kb and H-2Kk-expressing cells in FACS analysis (Bendelac, 1995). Cellular radioimmune assays have been used to confirm the binding of this antibody to spleen cells, and assess its strain specificity (Koch et al, 1983).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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