Mfg Part Number
Ab00883-1.32
Vector Labs Ab00883-1.32 Anti-PD-L2 [TY25], Mouse IgG1-D265A, Fc Silenced, kappa (100 μg)
Quick Overview
This antibody was raised by immunising SD rats with B7-DC-transfected RAW264.7 cells and selected on B7-DC-transfected NRK.
$692.90
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| Product Class: | Purified |
|---|---|
| Clone ID: | TY25 |
| Heavy Chain Modification: | Fc Silenced |
| Synonyms: | CD273; B7-DC; PDL2; B7DC; programmed death ligand 2; CD273; Pdcd1lg2 |
| Amount: | 100 μg |
| Application Codes Clone: | FC; IP; WB; Block |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds to mouse PD-L2 and competes with PD-1 binding. It does not cross-react with PD-L1. |
| Application Notes: | This antibody has been used to detect murine PD-L2 by flow cytometry on stimulated dendritic cells as well as various mouse tumour cells. It also detects immunoprecipitates mouse PD-L2 and can be used to detect it by non-reducing Western Blot. TY25 inhibits PD-L2 binding to PD-1 transfected cells (Ansari et al. 2003, PMID: 12847137) and thereby blocks PD-L2 : PD-1 signalling. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |