Mfg Part Number
Ab00883-2.3-VXB

Vector Labs Ab00883-2.3-VXB Anti-PD-L2 [TY25], Mouse IgG2a, Fc Silent™, kappa (100 mg)

Quick Overview
This antibody was raised by immunising SD rats with B7-DC-transfected RAW264.7 cells and selected on B7-DC-transfected NRK.
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Product Class:VivopureX
Clone ID:TY25
Heavy Chain Modification:Fc Silent™
Synonyms:CD273; B7-DC; PDL2; B7DC; programmed death ligand 2; CD273; Pdcd1lg2
Amount:100 mg
Application Codes Clone:FC; IP; WB; Block
Shipping Temperature:Wet Ice
Buffer Composition:PBS only.
Original Format:IgG2a
Storage Temperature:All VivopureX products are formulated in PBS only without addition of preservatives. To ensure optimal storage and prevent microbial contamination, only open and dispense under sterile conditions. Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody binds to mouse PD-L2 and competes with PD-1 binding. It does not cross-react with PD-L1.
Application Notes:This antibody has been used to detect murine PD-L2 by flow cytometry on stimulated dendritic cells as well as various mouse tumour cells. It also detects immunoprecipitates mouse PD-L2 and can be used to detect it by non-reducing Western Blot. TY25 inhibits PD-L2 binding to PD-1 transfected cells (Ansari et al. 2003, PMID: 12847137) and thereby blocks PD-L2 : PD-1 signalling.
More Information
Brand Vector Labs
UNSPSC 12161500