Vector Labs Ab00885-23.0 Anti-RAP tag [PMab-2], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: PMab-2
Synonyms: Podoplanin; E11 antigen epitope; Glycoprotein 38; Gp38; OTS-8; Ots8; RTI140; T1-alpha; T1A; Type I cell 40 kDa protein
Amount: 200 μg
Application Codes Clone: ELISA; FC; WB; IHC; epitope tag
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for amino acids 38-51 (GDDMVNPGLEDRIEC) of the platelet-aggregation-stimulating (PLAG) domain of rat podoplanin (PDPN). PDPN is a is a type I transmembrane protein that interacts with the platelet receptor C-type lectin-like receptor-2 (CLEC-2). PDPN is expressed by a wide variety of cells, including podocytes, lymphatic endothelial cells and type I alveolar cells. PDPN is also upregulated on certain cancer cells, where it mediates tumour-induced platelet aggregation by binding to CLEC-2 on platelets
Application Notes: The specificity of this antibody for endogenous rat podoplanin (PDPN) has been confirmed in ELISA, Western blot and flow cytometry analysis (Oki et al, 2015), where PMab-2 was shown not to react with human and mouse podoplanin. Flow cytometry has also been used to demonstrate the high binding affinity of this antibody (KD of PMab-2 = 5.9 × 10−10 M) (Oki et al, 2015). In immunohistochemical analysis, this antibody is highly sensitive to rat PDPN, detecting type I lung alveolar cells, colon lymphatic endothelial cells, podocytes and Bowman's capsule epithelial cells at low concentration (Oki et al, 2015). This antibody possess high affinity and specificity for the RAP epitope tag (DMVNPGLEDRIE), and can, therefore, be used to detect proteins in Western blots, flow cytometry and sandwich enzyme-linked immunosorbent assays (ELISA), as well as for protein purification. (Fujii et al, 2017).

Product Class:	Purified
Clone ID:	PMab-2
Synonyms:	Podoplanin; E11 antigen epitope; Glycoprotein 38; Gp38; OTS-8; Ots8; RTI140; T1-alpha; T1A; Type I cell 40 kDa protein
Amount:	200 μg
Application Codes Clone:	ELISA; FC; WB; IHC; epitope tag
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for amino acids 38-51 (GDDMVNPGLEDRIEC) of the platelet-aggregation-stimulating (PLAG) domain of rat podoplanin (PDPN). PDPN is a is a type I transmembrane protein that interacts with the platelet receptor C-type lectin-like receptor-2 (CLEC-2). PDPN is expressed by a wide variety of cells, including podocytes, lymphatic endothelial cells and type I alveolar cells. PDPN is also upregulated on certain cancer cells, where it mediates tumour-induced platelet aggregation by binding to CLEC-2 on platelets
Application Notes:	The specificity of this antibody for endogenous rat podoplanin (PDPN) has been confirmed in ELISA, Western blot and flow cytometry analysis (Oki et al, 2015), where PMab-2 was shown not to react with human and mouse podoplanin. Flow cytometry has also been used to demonstrate the high binding affinity of this antibody (KD of PMab-2 = 5.9 × 10−10 M) (Oki et al, 2015). In immunohistochemical analysis, this antibody is highly sensitive to rat PDPN, detecting type I lung alveolar cells, colon lymphatic endothelial cells, podocytes and Bowman's capsule epithelial cells at low concentration (Oki et al, 2015). This antibody possess high affinity and specificity for the RAP epitope tag (DMVNPGLEDRIE), and can, therefore, be used to detect proteins in Western blots, flow cytometry and sandwich enzyme-linked immunosorbent assays (ELISA), as well as for protein purification. (Fujii et al, 2017).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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