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Mfg Part Number

Ab00890-23.0-BS

Vector Labs Ab00890-23.0-BS Anti-alpha-V beta 3 integrin [EM22703], Rabbit IgG, kappa (1 mg)

VECTOR LABS

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Quick Overview

This antibody was raised by immunising rabbits, isolating B cells from the immunised rabbits and fusing these with a rabbit plasmacytoma cell line (established by Spieker-Polet et al., 1995) to produce stable hybridomas

$5,162.30

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Description

Product Class: Purified
Clone ID: EM22703
Synonyms: αvβ3; Vitronectin receptor subunit alpha; CD51; ITGB3; Platelet membrane glycoprotein IIIa; CD61; P06756; P05106; avb3
Amount: 1 mg
Application Codes Clone: IHC; WB; FC; ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS only.
Original Format: IgG
Storage Temperature: Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody is specific for the extracellular domain of human recombinant transmembrane-truncated (DTM) αvβ3 integrin, a dimeric cell surface protein that mediates cell attachment. αvβ3 integrin is also involved in tumour growth and angiogenesis.
Application Notes: This antibody has been used to specifically bind αvβ3 in Western blots and in flow cytometry, and to specifically stain αvβ3 integrins in FFPE (formalin fixed paraffin embedded)/TMA material, where murine antibodies function poorly (Goodman et al, 2012; Heß et al., 2014; Roth et al, 2013). αvβ3 was found to be strongly expressed in some invasive NSCLC samples, though was not expressed by PC3 prostate cells and human prostate tumours. In FC, the EM22703 signal is increased 3–10 fold in the presence of nanomolar concentrations of the antagonistic αvβ3 inhibitor cilengitide, indicating that EM22703 detects a ligand-induced binding site on αvβ3 (Goodman et al, 2012).

Product Class:	Purified
Clone ID:	EM22703
Synonyms:	αvβ3; Vitronectin receptor subunit alpha; CD51; ITGB3; Platelet membrane glycoprotein IIIa; CD61; P06756; P05106; avb3
Amount:	1 mg
Application Codes Clone:	IHC; WB; FC; ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS only.
Original Format:	IgG
Storage Temperature:	Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody is specific for the extracellular domain of human recombinant transmembrane-truncated (DTM) αvβ3 integrin, a dimeric cell surface protein that mediates cell attachment. αvβ3 integrin is also involved in tumour growth and angiogenesis.
Application Notes:	This antibody has been used to specifically bind αvβ3 in Western blots and in flow cytometry, and to specifically stain αvβ3 integrins in FFPE (formalin fixed paraffin embedded)/TMA material, where murine antibodies function poorly (Goodman et al, 2012; Heß et al., 2014; Roth et al, 2013). αvβ3 was found to be strongly expressed in some invasive NSCLC samples, though was not expressed by PC3 prostate cells and human prostate tumours. In FC, the EM22703 signal is increased 3–10 fold in the presence of nanomolar concentrations of the antagonistic αvβ3 inhibitor cilengitide, indicating that EM22703 detects a ligand-induced binding site on αvβ3 (Goodman et al, 2012).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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