Vector Labs Ab00897-13.12 Anti-CD35 [HB-8592], Human IgG4-S228P, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: HB-8592
Synonyms: CR1; C3BR; C4BR; HB8592; HB 8592; MoAb 543
Amount: 200 μg
Application Codes Clone: FC; IF; ELISA; IA assay; in vivo
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for Human CR1 but does not compete with clones 7G9 or E11; it binds to a site distinct from the C3b binding site and can be used to measure the number of receptors despite occupancy by ligand. This antibody cross-reacts with Chimpanzee, Baboon, Rhesus, and Cynomolgus monkey CR1. The scFv version of this antibody reacted with Mouse CR1.
Application Notes: This antibody has been used for the analysis of the expression of the CR1 receptor on macrophages isolated from the peritoneal effluents of CAPD patients with peritonitis and human leukemia-derived CD4⁺ T cell lines by flow cytometry (Hart et al., 1992; PMID: 1606734) (Yaskanin and Waxman, 1995; PMID: 7758124). This antibody was used in immune adherence (IA) binding and release assays (Edberg et al., 1992; PMID: 1376254). This antibody was used in in vivo clearance experiments (Taylor et al., 1997; PMID: 9000042). An scFv version of this antibody was generated, its pharmacokinetics were evaluated, and it was shown to bind mouse mixed lymphocytes and bone marrow-derived dendritic cells in an ELISA and to bind CR1-expressing mouse bone marrow-derived dendritic cells via immunofluorescence labeling (Knight et al., 2008; PMID: 18385459).

Product Class:	Purified
Clone ID:	HB-8592
Synonyms:	CR1; C3BR; C4BR; HB8592; HB 8592; MoAb 543
Amount:	200 μg
Application Codes Clone:	FC; IF; ELISA; IA assay; in vivo
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for Human CR1 but does not compete with clones 7G9 or E11; it binds to a site distinct from the C3b binding site and can be used to measure the number of receptors despite occupancy by ligand. This antibody cross-reacts with Chimpanzee, Baboon, Rhesus, and Cynomolgus monkey CR1. The scFv version of this antibody reacted with Mouse CR1.
Application Notes:	This antibody has been used for the analysis of the expression of the CR1 receptor on macrophages isolated from the peritoneal effluents of CAPD patients with peritonitis and human leukemia-derived CD4⁺ T cell lines by flow cytometry (Hart et al., 1992; PMID: 1606734) (Yaskanin and Waxman, 1995; PMID: 7758124). This antibody was used in immune adherence (IA) binding and release assays (Edberg et al., 1992; PMID: 1376254). This antibody was used in in vivo clearance experiments (Taylor et al., 1997; PMID: 9000042). An scFv version of this antibody was generated, its pharmacokinetics were evaluated, and it was shown to bind mouse mixed lymphocytes and bone marrow-derived dendritic cells in an ELISA and to bind CR1-expressing mouse bone marrow-derived dendritic cells via immunofluorescence labeling (Knight et al., 2008; PMID: 18385459).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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