Mfg Part Number
Ab00902-23.0
Vector Labs Ab00902-23.0 Anti-IL2R [AHT54], Rabbit IgG, kappa (200 μg)
Quick Overview
BALB/c mice were immunized with human T lymphoblasts and spleen cells of the immunized animals were fused with X63-Ag8.653 mouse myeloma cells. The supernatants of the resultant hybridomas were screened by a proliferation inhibition assay (inhibition of the human T lymphoblast response to IL-2). Two of those (the clones AHT54 and AHT107) that produced antibodies which inhibited IL-2 dependent proliferation of human T lymphoblasts were selected, cloned and recloned.
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| Product Class: | Purified |
|---|---|
| Clone ID: | AHT54 |
| Synonyms: | CD25; Interleukin 2 receptor; IL-2R; IL2 receptor; IL-2 receptor; Tac antigen; p55; AHT-54; AHT 54 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; FC; Block |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | AHT54 recognises a determinant close to the IL-2 binding site. |
| Application Notes: | AHT54 has been used in ELISAs for the detection of soluble human IL-2R in response to different forms of helminthic and protozoal infections (O. Josimovic et al. 1988). AHT54 has also been shown to have anti-proliferative activities, and chimeric AHT54 antibodies with a human contant region have an increased inhibitory effect on proliferation of T lymphocytes (Rose 1992). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |