Mfg Part Number
Ab00904-2.3
Vector Labs Ab00904-2.3 Anti-TCR [Desire-1], Mouse IgG2a, Fc Silent™, kappa (200 μg)
Quick Overview
mAb desire-1 was obtained after fusion of spleen cells from a BALB.K mouse immunised twice with KB5-C20 clone cells in RPMI 1640 medium, with non-Ig-producing myeloma cells X63-Ag8.653. Desire-1 was selected for its capacity to inhibit selectively the H-2Kb-specific cytolysis by clone KB5-C20.
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| Product Class: | Purified |
|---|---|
| Clone ID: | Desire-1 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | T cell receptor; T-cell receptor; Ti |
| Amount: | 200 μg |
| Application Codes Clone: | IP; FC; IF; crystallisation |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | mAb desire-1 recognises an epitope contributed by both the alpha and beta chains of the KB5-C20 TCR. |
| Application Notes: | Desire-1 has been used for the immunoprecipitation of soluble TCR molecules (Gregoire 1991 & Hua 1986). An FITC conjugated desire-1 has been used in flow cytometric analysis as a marker for clonotype expression (Schonrich 1991). Further, a Fab fragment derived from desire-1 has been used in the crystallisation of the T-cell receptor for 3D structure determination (Housset 1997). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |