Mfg Part Number
Ab00909-13.12
Vector Labs Ab00909-13.12 Anti-acetylcholine receptor [mAb198], Human IgG4-S228P, kappa (200 μg)
Quick Overview
This antibody was raised by immunising rats with acetylcholine receptor derived from human skeletal muscle.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | mAb198 |
| Synonyms: | nAchR; Neuronal acetylcholine receptor |
| Amount: | 200 μg |
| Application Codes Clone: | Crystallisation; WB; ELISA; IP; IHC; deplete |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 8344344 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the main immunogenic region of human acetylcholine receptor, which encompasses residues 67–76 of the extracellular region of the acetylcholine receptor α subunit. It cross-reacts with acetylcholine receptors from mammalian muscle, including bovine muscle, and from the electric organs of Torpedo rays and Electrophorus electric eels. |
| Application Notes: | The specificity of this antibody for the major immunogenic region of human acetylcholine receptor has been confirmed in Western blot and ELISA analysis (Barchan et al, 1998). This antibody cross-links acetylcholine receptors on the muscle surface and promotes their degradation; incubation of AChR-expressing cell lines with mAb198 results in a reduction in AChR (Barchan et al, 1988). Indeed, administration of this antibody to mice is sufficient to induce experimental autoimmune myasthenia gravis (EAMG), a neuromuscular disorder arising from a reduction in available acetylcholine receptors at the NMJ (Asher, 1993). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |