Mfg Part Number
Ab00915-10.3
Vector Labs Ab00915-10.3 Anti-DR5 [YSd1], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
Fab fragments were generated by constructing phage-display libraries in which the CDRs of a humanised Fab framework derived from the murine Ab 4D5 were randomised (see Eigenbrot et al, 1993). The 1st and 2nd CDRs of the VH domain were replaced by randomised sequences consisting only of Tyr and Ser residues, whilst the 3rd VH CDR was substituted with random loops of variable lengths comprising all naturally occurring amino acids. Specific Fabs were isolated using ELISA screening.
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| Product Class: | Purified |
|---|---|
| Clone ID: | YSd1 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | TRAILR2; Death receptor 5; DR-5; TRAIL receptor 2; Tumour necrosis factor receptor superfamily member 10B; TNFRSF10B |
| Amount: | 200 μg |
| Application Codes Clone: | NTRL |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 15854651 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | YSd1 was selected during phage display on the basis of its binding to the extracellular domain of DR5. It binds to the N-terminal portion of the molecule, binding to the smaller of two binding sites for the natural DR5 ligand Apo2L/TRAIL in the same fashion as the natural ligand. |
| Application Notes: | YSd1 bound DR5 with high affinity, shown using competitive phage ELISA as well as surface plasmon resonance (with a Kd of 34 nM). By binding to interfacial regions of DR5 which are sites of physiological ligand interaction, it is likely that YSd1 has the capacity to neutralise DR5 activity by inhibition of downstream signalling. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |