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Mfg Part Number

Ab00916-23.0

Vector Labs Ab00916-23.0 Anti-Kell [MIMA-8], Rabbit IgG, kappa (200 μg)

VECTOR LABS

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Quick Overview

Murine antibodies were produced by immunising BALB/c mice with the mouse erythroleukaemic (MEL) cell line stably expressing high levels of wildtype, full-length human Kell protein. This overexpression allowed several epitopes of Kell protein to serve as the immunogen, resulting in identification of several anti-Kell mAbs with different specificities, of which MIMA-8 targeted the Jsb antigen. Splenocytes were obtained from immunised mice and fused with the P3X63Ag8.653 myeloma cell line to produce a hybridoma.

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Description

Product Class: Purified
Clone ID: MIMA-8
Synonyms: CD238; Kell protein; Kell blood group glycoprotein homolog; Cluster of Differentiation 238
Amount: 200 μg
Application Codes Clone: FC
Shipping Temperature: Wet Ice
Origin Pub PMID: 10468879
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: MIMA-8 was shown to be specific for the Jsb epitope of the Kell protein using flow cytometry, whereby the mAb agglutinated Jsb+ , but not Jsa+Jsb-, erythrocytes (Chu et al, 1999). These erythrocytes also lacked other high-incidence Kell protein antigens. The Kell protein is a cell-surface metalloprotease, and constitutes one of the major blood antigenic systems of humans.
Application Notes: MIMA-8 was shown, by flow cytometry, to react to the Jsb epitope of Kell protein (Chu et al, 1999). As a result, MIMA-8 could be used as a diagnostic reagent for cell typing of patient blood samples, or as a research tool. Notably, the Jsb epitope is known to be located close to the Kell enzymatic active site, and thus MIMA-8 could be used to study the structure-function relationship at this region.

Product Class:	Purified
Clone ID:	MIMA-8
Synonyms:	CD238; Kell protein; Kell blood group glycoprotein homolog; Cluster of Differentiation 238
Amount:	200 μg
Application Codes Clone:	FC
Shipping Temperature:	Wet Ice
Origin Pub PMID:	10468879
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	MIMA-8 was shown to be specific for the Jsb epitope of the Kell protein using flow cytometry, whereby the mAb agglutinated Jsb+ , but not Jsa+Jsb-, erythrocytes (Chu et al, 1999). These erythrocytes also lacked other high-incidence Kell protein antigens. The Kell protein is a cell-surface metalloprotease, and constitutes one of the major blood antigenic systems of humans.
Application Notes:	MIMA-8 was shown, by flow cytometry, to react to the Jsb epitope of Kell protein (Chu et al, 1999). As a result, MIMA-8 could be used as a diagnostic reagent for cell typing of patient blood samples, or as a research tool. Notably, the Jsb epitope is known to be located close to the Kell enzymatic active site, and thus MIMA-8 could be used to study the structure-function relationship at this region.

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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