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Mfg Part Number

Ab00917-23.0

Vector Labs Ab00917-23.0 Anti-Envelope protein DIII [E111], Rabbit IgG, kappa (200 μg)

VECTOR LABS

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Quick Overview

Immunodeficient C57BL/6 background mice were infected with DENV-1 (genotype 2). In some cases, mice were boosted by injection of recombinantly produced DENV-1 E protein DIII. Splenocytes obtained from immunised mice were then fused with P3X63Ag8.53 myeloma cells to generate a hybridoma.

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Description

Product Class: Purified
Clone ID: E111
Synonyms: DENV1 DIII; Dengue virus 1 envelope protein DIII domain; DENV-1 envelope protein DIII domain; DENV-1 E protein DIII domain; DENV-1 E protein DIII
Amount: 200 μg
Application Codes Clone: NTRL; FC; WB; IP; IHC; IF
Shipping Temperature: Wet Ice
Origin Pub PMID: 23055922
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: DENV1-E111 was shown to bind the E protein DIII using yeast which specifically expressed each E protein domain (Shrestha et al, 2010). It was further shown that DENV1-E111 was specific for the type 1 DENV serotype, as well as genotype 2 of this serotype. Forward genetic screens could not identify DENV-1 DIII mutations which abrogated DENV1-E111 binding, however a crystal structure of a DENV1-E111 scFv comprising the VH and VL domains showed that the mAb bound the CC' loop of DIII (Austin et al, 2012). In the same study, the authors used SPR to elucidate an affinity of 18 nM for the DENV1-E111-DIII interaction.
Application Notes: DENV1-E111 has been shown to bind strongly to DENV-1 E protein DIII by immunostaining and flow cytometry (Shrestha et al, 2010). The mAb is a strong serotype- and genotype-specific binding agent, and so can be applied as a reliable diagnostic tool.

Product Class:	Purified
Clone ID:	E111
Synonyms:	DENV1 DIII; Dengue virus 1 envelope protein DIII domain; DENV-1 envelope protein DIII domain; DENV-1 E protein DIII domain; DENV-1 E protein DIII
Amount:	200 μg
Application Codes Clone:	NTRL; FC; WB; IP; IHC; IF
Shipping Temperature:	Wet Ice
Origin Pub PMID:	23055922
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	DENV1-E111 was shown to bind the E protein DIII using yeast which specifically expressed each E protein domain (Shrestha et al, 2010). It was further shown that DENV1-E111 was specific for the type 1 DENV serotype, as well as genotype 2 of this serotype. Forward genetic screens could not identify DENV-1 DIII mutations which abrogated DENV1-E111 binding, however a crystal structure of a DENV1-E111 scFv comprising the VH and VL domains showed that the mAb bound the CC' loop of DIII (Austin et al, 2012). In the same study, the authors used SPR to elucidate an affinity of 18 nM for the DENV1-E111-DIII interaction.
Application Notes:	DENV1-E111 has been shown to bind strongly to DENV-1 E protein DIII by immunostaining and flow cytometry (Shrestha et al, 2010). The mAb is a strong serotype- and genotype-specific binding agent, and so can be applied as a reliable diagnostic tool.

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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