Mfg Part Number
Ab00922-23.0
Vector Labs Ab00922-23.0 Anti-LHRH [SMI41], Rabbit IgG, kappa (200 μg)
Quick Overview
LHRH diacetate tetrahydrate was conjugated to potato acid phosphatase and an emulsion composed of the conjugate and Freunds complete adjuvant was injected into the hind limb of female CAF1 mice. Immune spleen cells were fused with P3-X63-Ag8.653/3 myeloma cells by a modification of the original cell fusion method using polyethylene glycol. A hybridoma screening assays were used to select for high affinity monoclonal antibodies.
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| Product Class: | Purified |
|---|---|
| Clone ID: | SMI41 |
| Synonyms: | GnRH; LHRH; LNRH; Anti-Gonadotropin-Releasing Hormone; GNRH1; Gonadoliberin; Gonadorelin; luteinizing hormone release hormone; SMI-41; SMI 41 |
| Amount: | 200 μg |
| Application Codes Clone: | IHC; IHC-P; RIA; therapeutic |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | SMI41 reacts with LHRH with an affinity of 10 pM and is specific for its C-terminal pentapeptide (G-L-R-P-G). |
| Application Notes: | SMI-41 has been used in immunocytochemistry experiments to detect the expression of mature LHRH in the brain detect cells containing mature LHRH peptide (Avigdor 2004). SMI-41 also has been expressed in mice via injection of a replication-incompetent, recombinant adeno-associated virus designed to express the antibody. This has been used to cofer long-term infertility in male and female mice (Li 2015). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |