Mfg Part Number
Ab00925-10.3
Vector Labs Ab00925-10.3 Anti-gH [YC15], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
Antibodies were produced from a murine hybridoma.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | YC15 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Glycoprotein H; Kaposi's sarcoma-associated herpesvirus glycoprotein H; KSHV glycoprotein H; KSHV gH; KSHV envelope glycoprotein H; Envelope glycoprotein H |
| Amount: | 200 μg |
| Application Codes Clone: | WB; NTRL; IHC; FC |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 21440007 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | YC15 was shown to bind ectopically-expressed gH on transfected cell lines, as well as neutralise infection by KSHV virions (unpublished observations). Kaposi's sarcoma is a rare type of cancer, characterised by neoplasmic growth, often associated with AIDS. |
| Application Notes: | The YC15 variable domain sequences were cloned and redesigned into the scFv format (Cai & Berger, 2011), and further fused to PE38, a construct of Pseudomonas aeruginosa exotoxin A which is lacking the N-terminal cell adhesion domain. In this format, YC15 showed specific dose-dependent binding to surface-expressed gH using flow cytometry. As an immunotoxin with the fused P. aeruginosa PE38 construct, YC15 facilitated potent cytotoxic activity based on recognition of surface gH epitopes. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |