Mfg Part Number
Ab00934-23.0

Vector Labs Ab00934-23.0 Anti-Shiga toxin 1 [5-5B], Rabbit IgG, kappa (200 μg)

Quick Overview
This antibody was raised by immunising mice with Stx1 toxoid.
$517.40
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Product Class:Purified
Clone ID:5-5B
Synonyms:Stx1; stx1B
Amount:200 μg
Application Codes Clone:WB; ELISA; neutralisation
Shipping Temperature:Wet Ice
Origin Pub PMID:15297734
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG1
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody is specific for the Stx1 B subunit, and does not recognise Stx2
Application Notes:This antibody has been shown to bind the B subunit of Stx1 in Western blot analysis (Nakao et al, 2002). This antibody is able to neutralise Stx1 cytotoxicity (Nakao, 2002). Flow cytometric experiments have revealed that this antibody binds to free Stx1 B subunits, not cell-bound Stx1, and inhibits Stx1 binding to its receptor on target cells (Nakao et al, 2002). A Fab derived from this antibody also specifically binds Stx1, as determined by ELISA, and neutralises Stx1 cytotoxicity (Inoue et al, 2004).
More Information
Brand Vector Labs
UNSPSC 12161500