Mfg Part Number
Ab00942-10.0
Vector Labs Ab00942-10.0 Anti-Envelope protein (soluble) [Z20], Human IgG1, kappa (200 μg)
Quick Overview
Anti-ZIKV mAbs were obtained from the sera and peripheral blood mononuclear cells of a ZIKV-infected patient.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | Z20 |
| Synonyms: | ZIKV sE; Zika virus soluble envelope protein; Zika virus envelope protein; ZIKV soluble envelope protein; ZIKV envelope protein; Zika virus sE protein; ZIKV sE protein; ZIKV sE; Z-20 |
| Amount: | 200 μg |
| Application Codes Clone: | NTRL; crystallisation; ELISA; FC; SPR |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 27974667 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | Z20 binds to the ZIKV soluble envelope (sE) protein, shown using SPR, kinetic competition assays and crystallography (Wang et al, 2016). This mAb is highly specific for ZIKV, but exhibited activity against DENV-1 to DENV-4 virions at very high concentrations. Shown crystallographically, Z20 binds almost exclusively to domain II of sE. ZIKV has recently become a major global health concern, following a 2015 outbreak. Viral infection is associated with neurological complications and microencephaly. |
| Application Notes: | Z20 was confirmed to bind ZIKV sE protein by ELISA, with an affinity of 0.16 uM shown by SPR (Wang et al, 2016). Using a FACS-based assay, it was shown that Z20 potently neutralised viral activity against infected C6/36 and Vero cells. In vivo, Z20 was able to protect 80% of IFN receptor KO mice from ZIKV infection. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |