Mfg Part Number
Ab00947-10.6-BS
Vector Labs Ab00947-10.6-BS Anti-HLA-A2/A28 [3PF12], Human Fab fragment, His-Tagged, kappa (1 mg)
Quick Overview
A 57-year-old female presented with post-transfusion purpura 5 days following a blood transfusion. Serological studies with the sera of this patient identified the presence of anti-HLA-A2. A 50-ml EDTA anti-coagulated blood sample was obtained from the patient in the acute phase of her PTP, and mononuclear cells were isolated from the blood sample by layering onto a Ficoll gradient. An oligo dT primer was used to direct first strand cDNA synthesis and IgG encoded VH was amplified and an scFv library was constructed. Phage particles expressing anti-HLA A2/A28 scFvs were selected by panning.
$5,162.30
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| Product Class: | Purified |
|---|---|
| Clone ID: | 3PF12 |
| Heavy Chain Modification: | His-Tagged |
| Synonyms: | Human Leukocyte antigen; HLA-A2; HLA-A28; HLA A2; HLA A28 |
| Amount: | 1 mg |
| Application Codes Clone: | IF; FC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS only. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | Amino acid residues 142-145 (TTKH) of the alpha2 domain of the heavy chain of the HLA class I molecules are most likely to be involved in the formation of the epitope recognised by the scfv. |
| Application Notes: | ScFv 3PF12 has been used in immunofluorescence and flow cytometry experiments to test antibody binding to HLA-A2 positive lymphocytes and platelets (Watkins 2000). The scFv has been tested in in vitro assays and have been shown to inhibit target cell killing (Watkins 2003). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |