Mfg Part Number
Ab00948-10.3
Vector Labs Ab00948-10.3 Anti-Envelope protein DIII [2D73], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
BALB/c mice were immunised with recombinantly-produced envelope protein domain III (EDIII) from DENV-3. Splenocytes were obtained from immunised mice and fused with the NS-1 myeloma cell line to generate hybridomas.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 2D73 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | DENV E DIII; Dengue virus envelope protein domain III; DENV envelope protein domain III; 2D-73 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; crystallisation |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | 2D73 reacts with residues 309-319 (DKEMAETQHGT) within domain III of the DENV envelope protein, shown crystallographically (Li et al, unpublished). Although raised against DENV-3 EDIII, 2D72 cross-reacts with EDIII from all four DENV serotypes. 2D73 does not react with other DENV-related flaviviruses, such as WNV, YFV and JEV. |
| Application Notes: | 2D73 was able to strongly neutralise DENV infection in a serotype-independent manner, shown using both ELISAs and ELISPOT-based assays (Li et al, 2013). 2D73 has also been used, as a Fab fragment, to crystallise the DENV-4 EDIII protein (Li et al, unpublished). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |