Mfg Part Number
Ab00949-1.1
Vector Labs Ab00949-1.1 Anti-JAML [HL4E10 ], Mouse IgG1, lambda (200 μg)
Quick Overview
An Armenian hamster was immunised with the 7-17 dendritic epidermal T cell line. Splenocytes were obtained from immunised hamsters and fused with the X63Ag8.653 myeloma cell line to generate a hybridoma.
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| Product Class: | Purified |
|---|---|
| Clone ID: | HL4E10 |
| Synonyms: | MICA1; Junctional adhesion molecule-like; Adhesion molecule interacting with CXADR antigen 1; Dendritic cell-specific protein CREA7-1 |
| Amount: | 200 μg |
| Application Codes Clone: | IP; FC; IF; FACS; crystallisation; SPR |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 21220118 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | HL4E10 was found to be specific for JAML, as this protein was identified as the ligand for the mAb from immunoprecipitation (Witherden et al, 2010). Biochemical binding studies showed that HL4E10 bound the JAML D2 Ig domain, which was corroborated with a recent crystal structure of the JAML ECD and a HL4E10 Fab fragment (Verdino et al, 2011). HL4E10 has an affinity for JAML of 8 nM, characterised by SPR. |
| Application Notes: | HL4E10 has been used to detect JAML by both IP and FC (Witherden et al, 2010). IF labelling showed that JAML colocalises with the T cell receptor. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |