Mfg Part Number
Ab00950-10.0
Vector Labs Ab00950-10.0 Anti-CD10 [NL-1], Human IgG1, kappa (200 μg)
Quick Overview
BALB/c mice were immunised with acute lymphocytic leukaemia cells from a human leukaemia patient. Splenocytes were obtained from immunised mice and fused with MOPC-21 NS/1 myeloma cells to generate a hybridoma.
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| Product Class: | Purified |
|---|---|
| Clone ID: | NL-1 |
| Synonyms: | Neprilysin; Cluster of Differentiation 10; Membrane metallo-endopeptidase; MME; Neutral endopeptidase 24.11; Neutral endopeptidase; NEP; Common acute lymphocytic leukaemia antigen; CALLA; Skin fibroblast elastase; SFE; CMT2T; SCA43; Atriopeptidase; Enkephalinase |
| Amount: | 200 μg |
| Application Codes Clone: | IP; WB; IF |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody reacts with human CD10. CD10 is a widely-expressed cell-surface metalloprotease, responsible for the cleavage of multiple peptide hormones at target tissues. CD10 is also a common marker for acute lymphocytic leukaemia. |
| Application Notes: | NL-1 was shown to react with several leukaemic cell lines using immune adherence assays (Ueda et al, 1982). These authors also confirmed that NL-1 is specific for CD10 using IP. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |