Mfg Part Number
Ab00953-23.0
Vector Labs Ab00953-23.0 Anti-DR4 [mAY4], Rabbit IgG, lambda (200 μg)
Quick Overview
mAb mAY4 was generated by immunising BALB/c mice with the ECD (residues 1-215) of human DR4. Splenocytes were obtained from immunised mice and fused with murine Sp2/O myeloma cells. mAY4 was cloned by RT-PCR and reformatted as an scFv.
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| Product Class: | Purified |
|---|---|
| Clone ID: | mAY4 |
| Synonyms: | CD261; Death receptor 4; Tumor necrosis factor receptor superfamily member 10A; TNF-related apoptosis-inducing ligand receptor 1; TRAIL receptor 1; TRAILR1; TRAIL-R1; TRAILR 1; TNFRSF10A; APO2 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; SPR; WB |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Chicken scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | mAY4 was shown to bind DR4 by ELISA (Sung et al, 2009). These experiments also showed that mAY4 did not react with other homologous death receptors, including DR5, decoy receptor (DcR) 1, DcR2, FAS receptor, and mTR (a mouse ortholog of human DR4 and DR5). SPR showed an affinity of 7.1 nM for interaction with DR4. mAY4 bound to a different epitope on DR4 than the physiological ligand TRAIL. DR4 is a pro-apoptotic receptor expressed on all cells. It represents a promising target for anti-cancer therapeutic agents. |
| Application Notes: | mAY4 was shown to bind DR4, but not other related homologs, using ELISAs and SPR (Sung et al, 2009). The mAb and scFv-reformatted version were also used in cell viability assays for assessment of tumouricidal activity. More recently, the mAY4 CDRs were humanised and expressed as an scFv-reformatted antibody fragment (Lee et al, 2010), although the humanised version had a 10-fold lower affinity for DR4. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |