Mfg Part Number
Ab00955-10.3
Vector Labs Ab00955-10.3 Anti-Asialoglycoprotein receptor [C2], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
A human non-immune phage antibody library was screened with the recombinant carbohydrate recognition domain of rCRDH1 (the H1 subunit of human asialoglycoprotein receptor). Anti-rCRDH1 phage clones were obtained after 4 rounds of screening with rCRDH1-coated immunotubes and single positive colonies were further selected with the expressed thioredoxin-His-S tag of the wild-type pET32c. Specific anti-rCRDH1 phage clones were transfected into E. coli HB2151 and induced for secreted expression of scFv antibody.
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| Product Class: | Purified |
|---|---|
| Clone ID: | C2 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | ASGPR; ASGP-R; ASGR1; C-Type Lectin Domain Family 4 Member H1; Hepatic Lectin H1; CLEC4H1; HL-1; |
| Amount: | 200 μg |
| Application Codes Clone: | IHC; WB; therapeutic |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | C2 has been found to recognise denatured and native forms of asialoglycoprotein receptor. |
| Application Notes: | C2 has been utilised in Western Blot and immunohistochemical experiments in order to establish its ability to bind to denatured ASGPR (Cao 2006). Since C2 is able to recognise denatured and native forms of the ASGPR, the antibody could potentially be used to target molecules for gene therapy of hepatocellular carcinoma or other liver diseases (Cao 2006). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |