Mfg Part Number
Ab00973-1.69
Vector Labs Ab00973-1.69 Anti-TIGIT [4D4], Mouse Bispecific, anti-mPD-L1, Bispecific antibody, kappa (50 μg)
Quick Overview
Armenian hamsters were immunized with recombinant mouse TIGIT tetramers by a combination of s.c. and food pad immunization and booster injections. Draining lymph nodes were fused with Sp2/0-Ag14 cells, selected in HAT (hypoxanthine/aminopterin/thymidine) medium and supernatants were screened for specificity by ELISA and flow cytometry using TIGIT-transfectants.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 4D4 |
| Heavy Chain Modification: | Bispecific antibody |
| Synonyms: | VSIG9; T Cell Immunoreceptor With Ig And ITIM Domains; V-Set And Immunoglobulin Domain Containing Protein 9; V-Set And Transmembrane Domain Containing Protein 3; VSTM3; T Cell Immunoreceptor With Ig And ITIM Domains; Washington University Cell Adhesion Molecule; WUCAM |
| Amount: | 50 μg |
| Application Codes Clone: | Agonist; FC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This is a bispecific anti-mouse antibody created by fusing anti-PDL-1 VHH domains to the C-terminus of the heavy chains) of the anti-mouse TIGIT antibody clone 4D4 in a mouse IgG1 D265 format. The mouse IgG1 D265 a format is an Fc-silenced format, prevent |
| Specificity Statement: | 4D4 specifically recognises TIGIT. |
| Application Notes: | 4D4 is an agonistic anti-TIGIT antibody, and addition of plate bound 4D4 to anti-CD3/anti-CD28 stimulated T cells was shown to inhibit their proliferation (Joller 2011). Further, 4D4 has been used in order to identify genes that are downstream of TIGIT signaling in Tregs, as TIGIT engagement might modulate the Treg phenotype in tumor tissue (Liu 2016). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |