Mfg Part Number
Ab00973-2.0
Vector Labs Ab00973-2.0 Anti-TIGIT [4D4], Mouse IgG2a, kappa (200 μg)
Quick Overview
Armenian hamsters were immunized with recombinant mouse TIGIT tetramers by a combination of s.c. and food pad immunization and booster injections. Draining lymph nodes were fused with Sp2/0-Ag14 cells, selected in HAT (hypoxanthine/aminopterin/thymidine) medium and supernatants were screened for specificity by ELISA and flow cytometry using TIGIT-transfectants.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 4D4 |
| Synonyms: | VSIG9; T Cell Immunoreceptor With Ig And ITIM Domains; V-Set And Immunoglobulin Domain Containing Protein 9; V-Set And Transmembrane Domain Containing Protein 3; VSTM3; T Cell Immunoreceptor With Ig And ITIM Domains; Washington University Cell Adhesion Molecule; WUCAM |
| Amount: | 200 μg |
| Application Codes Clone: | Agonist; FC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Hamster format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | 4D4 specifically recognises TIGIT. |
| Application Notes: | 4D4 is an agonistic anti-TIGIT antibody, and addition of plate bound 4D4 to anti-CD3/anti-CD28 stimulated T cells was shown to inhibit their proliferation (Joller 2011). Further, 4D4 has been used in order to identify genes that are downstream of TIGIT signaling in Tregs, as TIGIT engagement might modulate the Treg phenotype in tumor tissue (Liu 2016). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |