Mfg Part Number
Ab00982-13.12
Vector Labs Ab00982-13.12 Anti-Hemin [1D3], Human IgG4-S228P, kappa (200 μg)
Quick Overview
This antibody was raised against a hemin-human immunoglobulin in BALB/c mice.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 1D3 |
| Synonyms: | Panhematin; Hematin |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; IF; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgM |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | The antibody is specific to metal-substituted protoporphyrin. CDRH2 plays an essential role in recognition. The antibody has the highest affinity for PP[Fe]-PEG and a five-times lower affinity for PP[Pd]-PEG, PP[Zn]-PEG, and PP[Ga]-PEG. |
| Application Notes: | The affinity of this antibody for hemin has been characterized by ELISA (Uda et al., 1993). The affinity constant of the antibody and individual CDR segments has been examined with fluorescence measurement of protoporphyrin-PEG binding (Hifumi et al., 1999). The mouse IgM version of this antibody (Ab00982-21.0) was used as a primary antibody for immunocytochemistry (IHC) in myelinating cultures after treatment with hemin/FeCl3 (Baldacchino et al., 2022; PMID: 35569511). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |