Mfg Part Number
Ab00992-13.12
Vector Labs Ab00992-13.12 Anti-C5a [Fab 100], Human IgG4-S228P, kappa (200 μg)
Quick Overview
A panel of mAbs against the activated complement component C5a was obtained from a filamentous phage M13-Fab display library generated from mice immunized with human rC5a. Fabs isolated from the library after iterative selection against rC5a bound to both rC5a and purified C5. To isolate Fabs specific for neoepitopes expressed on C5a but not on the native complement component C5, the library was rescreened in a competitive manner. The phage Fab library was first incubated with immobilized C5 to deplete C5 reactive Fabs. The C5 nonadherent phage were then incubated with immobilized rC5a in the presence of soluble C5. Bound phage were eluted and subjected to two additional cycles of subtraction with immobilized C5 and selection with immobilized rC5a in the presence of soluble C5. After three cycles of this competitive biopanning, Fabs reactive with rC5a were isolated.
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| Product Class: | Purified |
|---|---|
| Clone ID: | Fab 100 |
| Synonyms: | Complement Component 5a; Complement C5; C3 and PZP-Like Alpha-2-Macroglobulin Domain Containing Protein 4; Complement Component 5; C5a Anaphylatoxin; Prepro-C5; CPAMD4; Anaphylatoxin C5a Analog; ECLZB; C5D; C5b |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 8157971 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse Fab format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | Fab 100 recognises both C5 and C5a. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |