Mfg Part Number
Ab01001-23.0
Vector Labs Ab01001-23.0 Anti-Ganoderic acid A [12 A], Rabbit IgG, kappa (200 μg)
Quick Overview
BALB/c mice were immunised by injection of ganoderic acid A-BSA conjugates. Splenocytes were obtained from immunised mice and fused with SP2/0 murine myeloma cells to generate hybridomas.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 12 A |
| Synonyms: | GAA; 12A; 12-A |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | mAb 12 A was shown to react with ganoderic acid A using indirect and competitive ELISAs (Sakamoto et al, 2016). It was also shown that mAb 12 A did not cross react significantly with 32 other types of Ganoderma triterpenoids. Ganoderic acid A is one of the main bioactive triterpenoids in the Ganoderma genus of medicinal mushrooms, traditionally used in medicine. |
| Application Notes: | mAb 12 has been used in both indirect and competitive ELISAs, using 33 different types of Ganoderma triterpenoid including ganoderic acid A (Sakamoto et al, 2016). More recently, mAb 12 has been reformatted as a scFv fragment and applied in ELISAs for detection of ganoderic acid A (Sakamoto et al, unpublished). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |