Mfg Part Number
Ab01005-23.0
Vector Labs Ab01005-23.0 Anti-Fusarium [FvCA4], Rabbit IgG, lambda (200 μg)
Quick Overview
White leghorn chickerns were immunised by intramuscular injection of a preparation of cell wall-bound proteins isolated from the wh1-2 fungus strain of F. verticilliodes. Splenocytes were obtained from immunised chickens and used for RNA extraction and cDNA synthesis.
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| Product Class: | Purified |
|---|---|
| Clone ID: | FvCA4 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB; IF |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 22837678 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Chicken scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | FvCA4 was shown to exhibit a high affinity for F. verticilliodes cell wall-bound proteins using ELISA with both soluble antibody fragment or phage-expressed antibody (Hu et al, 2012). Again shown by ELISA, FvCA4 cross-reacted with several other Fusarium species, but not with non-Fusarium fungi. |
| Application Notes: | FvCA4 was used in ELISAs, both as soluble scFv fragments and as phage-expressed antibodies, for the detection of purified F. verticillioides cell wall-bound proteins and Fusarium species from contaminated maize grains. In addition, cell wall-bound proteins were also detected by immunoblotting from several fungal species, not limited to the Fusarium genus. Immunofluorescent labelling showed that FvCA4 localised to the cell surface of F. verticillioides conidiospores and hyphae. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |