Mfg Part Number
Ab01043-2.3
Vector Labs Ab01043-2.3 Anti-CD45 (leukocyte common antigen) [YBM/42.2.2], Mouse IgG2a, Fc Silent™, kappa (200 μg)
Quick Overview
This antibody was raised by immunising rats with CBAfiCaH mouse bone marrow cells.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | YBM/42.2.2 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | PTPRC; YBM42; YBM/42; YBM42.2.2; Receptor-type tyrosine-protein phosphatase C; L-CA; CD45 |
| Amount: | 200 μg |
| Application Codes Clone: | IP; RIA; FC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognises a cell surface marker present on all leukocytes but absent from erythroid cells. |
| Application Notes: | Radioactive binding assay tests demonstrated the ability of the YBM/42.2.2 antibody to label mature hemopoietic cells and a variety of cell lines which were neoplastic representatives of progenitor cells (Watt et al., 1983). This antibody was also used to sort CBA bone marrow cells via FACS and the results confirmed the specificity profile acuired in RIA experiments (Watt et al., 1983). Similar, FACS analysis was performed on mouse fetal liver cells as well (Boehm et al., 2014;Watt et al., 1983). Finally, it cao identify leukocytes and is also recommended to enrich for early progenitor cells from mouse fetal liver (which is predominantly populated by erythroid line) (Watt et al., 1983). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |