Vector Labs Ab01056-1.32 Anti-CD70 [TAN1-6], Mouse IgG1-D265A, Fc Silenced, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: TAN1-6
Heavy Chain Modification: Fc Silenced
Synonyms: TNFSF7; CD27 ligand; CD27-L; Cd27l; CD27LG; CD70 antigen; Ki-24 antigen; Tnfsf7; tumor necrosis factor (ligand) superfamily, member 7; Tumor necrosis factor ligand superfamily member 7
Amount: 200 μg
Application Codes Clone: FC; WB; IF; blocking
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for murine CD70, a 30-33 kDa type II transmembrane glycoprotein. CD70 is a member of the TNF family and is expressed by activated mouse T and B lymphocytes. Interaction of CD70 with CD27, its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.
Application Notes: This antibody has been used in various FACS analyses to reveal a critical role for CD70 in CD8 T cell priming by CD40-licensed antigen-presenting cells (Taraban et al, 2004), to demonstrate the differential requirement for CD70 and CD80/CD86 in dendritic cell-mediated activation of tumor-tolerized CD8 T cells (Bak et al, 2012), to examine how temporal changes in dendritic cell subsets, cross-priming and costimulation via CD70 control CD8(+) T cell responses to influenza (Ballesteros-Tato et al, 2010), and to show that CD70 is downregulated by interaction with CD27 (Kuka et al, 2013). In addition, this antibody has been used in vivo to block the CD70-CD27 interaction as part of the two studies examining how CD70 is critically required for the priming of CD8+ T cells (Taraban et al, 2004; Taraban et al, 2006).

Product Class:	Purified
Clone ID:	TAN1-6
Heavy Chain Modification:	Fc Silenced
Synonyms:	TNFSF7; CD27 ligand; CD27-L; Cd27l; CD27LG; CD70 antigen; Ki-24 antigen; Tnfsf7; tumor necrosis factor (ligand) superfamily, member 7; Tumor necrosis factor ligand superfamily member 7
Amount:	200 μg
Application Codes Clone:	FC; WB; IF; blocking
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for murine CD70, a 30-33 kDa type II transmembrane glycoprotein. CD70 is a member of the TNF family and is expressed by activated mouse T and B lymphocytes. Interaction of CD70 with CD27, its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.
Application Notes:	This antibody has been used in various FACS analyses to reveal a critical role for CD70 in CD8 T cell priming by CD40-licensed antigen-presenting cells (Taraban et al, 2004), to demonstrate the differential requirement for CD70 and CD80/CD86 in dendritic cell-mediated activation of tumor-tolerized CD8 T cells (Bak et al, 2012), to examine how temporal changes in dendritic cell subsets, cross-priming and costimulation via CD70 control CD8(+) T cell responses to influenza (Ballesteros-Tato et al, 2010), and to show that CD70 is downregulated by interaction with CD27 (Kuka et al, 2013). In addition, this antibody has been used in vivo to block the CD70-CD27 interaction as part of the two studies examining how CD70 is critically required for the priming of CD8+ T cells (Taraban et al, 2004; Taraban et al, 2006).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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